Once a gene had been identified to be placed into another organism, it can be cut from DNA using a restriction enzyme and then placed in a vector.
The vast majority of genetic engineering uses bacterial plasmids as the vector.
A plasmid is a small circular piece of DNA.
Plasmids are found in many types of bacteria and are separate from the main bacteria chromosomes.
Plasmids often carry genes that code for resistance to antibiotic chemicals.
If plasmids are cut with the same restiction enzyme as that used to isolate the gene, then complementary stick ends will be formed. Mixing quantities of plasmid and gene in the presence of ligase enzyme means that some plasmids will combine witht the gene, which then becomes sealed into the plasmid to form a recombinant plasmid.
It is important to remember that many cut plasmds will, in the presence of liase enzyme, simply reseal to reform the original plasmid.
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