copying genes and inserting them into other organisms is known as genetic engineering or genetic modification and its the first stage of gene therapy
Restriction enzymes endonucleases are used to chop up healthy DNA, cutting them at specific sites in small pieces which can be handled more easily
each restrictive endonuclease will only cut the DNA at a specific (restricted) site within the DNA sequence
some restriction enzymes cut the DNA so it leaves a couple of base pairs known as a sticky end which enables other DNA to be attached
Artificial copies of healthy genes can also be made via taking mRNA molules transcribed from the gene and using the enzyme reverse transcriptase to make complementary base pairs
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vectors
next stage in gene therapy is to attatch another piece of DNA to the gene this is called a vector which will carry the gene to its target cell
Plasmids the circular strand of DNA found in bacteria are often used as vectors- once the plasmid gets into the hst cell it can combine its DNA with the cell to form recombinant DNA
they are needed to carry new DNA into a human cell
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CF and vectors
for CF harmless viruses and liposomes (spheres from lipid bilayer) are used , the virus infects the lung epithelials cells and inserts the viral DNA included the added gene
liposomes fuse with the cell membrane and can pass through and diliver the new DNA into the cytoplasm
at the moment the virus is the most effective way of transporting the gene
specific markers can be added with the new gene to make insertion of the gene easier
Once the healthy DNA is inside the lung epithelials the healthy gene should be transcribed and translated producing a normal active CFTR protein releaving some of the symptoms
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