active site is less effected by temperature or pH change, therefore less likely to change shape- more reactions
Disadvantages
active site less easily available- rate of reaction slower as it takes longer for substarte to reach the active site
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enaymes- temperature
if temperature gets tooo high, there is more kinetic energy, so the enzymes vibrate more causing hydrogen bonds to break, causing distruption to the tertiary structure, thwerefore denaturing the enzyme (active site changes the shape)
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why do we use a control in the experiment
control- is to check that it is the enzyme responsible for the reaction
to test this
- you test without the enzyme
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relpication
why do we do them
to make them more reliable
identify any annomalies
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intervals
why are small intervals better?
easier to confirm the trend of the reaction easier to wokr out the true value
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syringe
use 1cm3 syringe for water- accurate when measuring
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