Enzymes - measure how a fast a product appears

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Step 1

The enzyme catalase catalyses the breakdown of hydrogen peroxide into water and oxygen

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Step 2

You can collect the oxygen and measure how much is produced in a set time

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Step 3

Use a pipette to add a set amount of hydrogen peroxide to a boiling tube. Put the tub in a water bath at 10 ºC.

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Step 4

Set up the rest of the apparatus as shown. Add a source of catalase (e.g. 1cm3 of potato) to the hydrogen peroxide and quickly attach the bung

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Step 5

Record how much oxygen is produced in the first minute. Repeat three times and calculate the mean

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Step 6

Repeat at 20 ºC, 30 ºC and 40 ºC. 

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Step 7

Control any variables (e.g. pH, the potato used, the size of the potato pieces, etc.) to make it a fair test

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Step 8

Calculate the mean rate of reaction at each temperature by dividing the mean volume of oxygen produced (in cm3) by the time taken (i.e. 60 s). The units will be cm3/second.

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