The enzyme catalase catalyses the breakdown of hydrogen peroxide into water and oxygen.
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Step 2
You can collect the oxygen and measure how much is produced in a set time.
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Step 3
Use a pipette to add a set amount of hydrogenperoxide to a boilingtube. Put the tub in a water bath at 10 ºC.
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Step 4
Set up the rest of the apparatus as shown. Add a source of catalase (e.g. 1cm3 of potato) to thehydrogenperoxideandquicklyattach the bung.
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Step 5
Record how much oxygen is produced in the firstminute. Repeatthreetimes and calculate the mean.
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Step 6
Repeat at 20 ºC, 30 ºC and 40 ºC.
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Step 7
Control any variables (e.g. pH, the potato used, the size of the potato pieces, etc.) to make it a fair test.
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Step 8
Calculate the mean rate of reaction at each temperature by dividing the mean volume of oxygenproduced (in cm3) by thetime taken(i.e. 60 s). The units willbecm3/second.
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