Enzymes

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Properties of Enzymes

  • biological catalysts
  • proteins
  • control the chemical reactions taking place in every cell
  • all enzymes are specific and can only catalyse one type of molecule
  • optimum pH
  • optimum temperature
    • if the temperature is higher or lower, the enzymes will catalyse the molecule at a much slower rate
    • if the temperature is too high, the enzyme is denatured
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Lock and Key Model

  • a substrate is held in an active site, increasing the probability that a reaction will take place
    • enzyme and substrate
    • enzyme-substrate complex
      • the enzyme is specific to a certain substrate
    • enzyme and products
      • enzyme unchanged at end of reaction
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Biological Washing Powder

  • contain digestive enzymes, allowing clothes to be washed at comparatively lower temperatures uses less energy
    • lipase breaks down fats to fatty acids and glycerol
    • protease breaks down proteins into amino acids
    • carbohydrase breaks down starch to glucose
  • uses less energy
  • some people have allergies
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Effect of pH on amylase (carbohydrase enzyme)

  • three equal sized hole cut into agar jelly containing starch
  • each hole is filled with the same volume and same concentration of a different enzyme solution left at 20 C
  • after 30 minutes the petri dish is flooded with iodine solution
  • 1% amylase at pH2
    • agar around hole stays black as the starch is not digested
    • pH is too low for amylase
  • 1% amylase at pH7
    • starch agar around hole becomes clear as starch has been digested
    • optimum pH
  • 1% boiled, cooled amylase
    • agar around hole stays black as starch is not digested
    • amylase has been denatured
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