Background info: Beta-galactosidase (lactase) is an enzyme that breaks down lactose into glucose and galactose. Pouring milk through a column of immobilised lactase wil result in the enzyme breaking down the lactose.
- Add 8cm3 of sodium alginate solution (2%) to a small beaker
- Add 2cm3 of lactase to the beaker
- Add one drop of food colouring to allow the reaction to be seen more clearly
- Mix thoroughly keeping bubbles to a minimum
- Draw this mixture into a barrel of a 10cm3 plastic syringe
- Add 1.5g of calcium chloride to 100cm3 of distilled water into 250cm3 beaker
- Drop the enzyme from the syringe into the calcium chloride solution. Allow the immobilised enzyme beads that start to form to harden for about 10mins
- Remove the beads and rinse thoroughly with water
- Rinse the syringe and remove the plunger and fix the barrel to a retort stand
- Place a small piece of gauze near the tip of the syringe to prrevent the beads from blocking the syringe nozzle
- Add the beads to the syringe
- Test the milk after it has been filtered through the beads with Clinistix (Pink →Blue)
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