Mary Stuart (Mary Queen of Scots) - Descendant from Henry VIII, therefore a threat to Elizabeth's reign. She was a Catholic tudor, therefore highly supported by the majority of people in the country. Mary also believed that she was the rightful monarch after being kicked off of her throne in Scotland. She was a monarch in her own right and had international recognition, which was hard for Elizabeth as she was not just some rebel that Elizabeth could imprison and execute. Philip of Spain was also a Catholic and potential father-in-law to Mary, which also caused issues as he was an international power and supporter of Mary.
The Northern Rebellion - Caused by wanting Mary on the throne. It was run by the nobility (earls) in 1569. Sussex and Leicester factions.
Marian exiles - Extreme Protestants, opposed Elizabeth even though she was a Protestant herself. Came through from Mary's reign when they fled to extreme Protestant states (Germanic). They made Elizabeth change her title to 'Supreme Governess' as they were not happy with women taking the title of God.
No heir - Elizabeth was without a husband and she was heirless. No Tudor to pass the throne down to.
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