DNA & RNA DNA, RNA, translation, transcription 3.0 / 5 based on 2 ratings ? BiologyDNA, genetics and evolutionA2/A-levelAll boards Created by: catherine spencerCreated on: 12-04-12 13:21 differences between DNA & RNA DNA has bases GCAT, RNA has bases GCAU. DNA has two nucleotide strands, RNA has one nucleotide strand. DNA has deoxyribose sugar, RNA has ribose sugar. DNA has more nucleotides in chain than RNA. DNA is helical, RNA is either straight or clover shaped. 1 of 8 Differences between mRNA & tRNA mRNA has no hydrogen bonds but tRNA does. mRNA has a bigger nucleotide chain than tRNA. mRNA has a straight chain. tRNA is clover shaped. mRNA doesn't have a binding site, tRNA has a binding site for an amino acid. 2 of 8 Transcription DNA unwinds at the point where a gene is to be used. Hydrogen bonds break so the strands of DNA seperate, exposing the bases. RNA nucleotides complementary base pair with the sense strand. A single chain of RNA nucleotides which are complementary to the DNA has been produced. RNA polymerase joins the ribose-phosphate backbone to form a molecule of RNA. The molecule created is mRNA. 3 of 8 post transcriptional processing of mRNA The molecule of mRNA that has been produced is edited before it leaves the nucleus. non-coding intron sequences are removed. The coding sequences are then spliced back together. 4 of 8 Translation (1) mRNA travels from the nucleus to the cytoplasm. mRNA binds to a ribosome. two mRNA codons are exposed on the ribosome. A tRNA carrying a specific amino acid binds to the mRNA. this is due to complementary base pairing. A 2nd tRNA-amino acid complex binds to the 2nd codon on the mRNA. Condensation reaction takes place between the two amino acids, forming a peptide bond. 5 of 8 Translation (2) Ribosome moves along the mRNA to the next codon. 1st tRNA goes back to the cytoplasm to pick up another amino acid. The process continues until a stop codon is reached. The polypeptide terminates here. The polypeptide is released into the cytoplasm or the lumen of the E.R. 6 of 8 The genetic code ... Is universal - the same for all organisms. Is degenerate - There are at least two possible codons for many amino acids. Is non-overlapping - Each base is part of one codon only. 7 of 8 Gene mutation A change in the base sequence carried by DNA. Mutagenic agents e.g uv radiation, x rays and cigarette smoke trigger mutations. Alterations to DNA may have major effects on the shape and function of a protein. A mutation may cause no change (because the code is degenerate). A change to one triplet ( when substitution takes place). A change to several triplets (addiition/deletion causing a frameshift). 8 of 8
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