DNA Hybridisation

DNA Hybridisation

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  • Created by: Rosie
  • Created on: 12-01-12 15:14

Mutations

Mutations

Due to mutations, the sequence of two organisms with a common ancestor will become more dissimular over time.

One way to determine simularities in the DNA of different organisms is by using DNA hybridisation.

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DNA hybridisation

DNA hybridisation

  • DNA from 2 species is extracted and purified.
  • DNA from one of the species is labelled using radioactive or fluorescent marker to it.
  • Both sets of DNA is heated to seperate the strands (removing H bonds)
  • The mixture is cooled to allow the strands to combine with strands with complementary organic bases.
  • Some double strands will have one strand of each species (one labelled, one unlabelled.)
  • These hybrid strands are seperated from the mixture.
  • These are heated at different stages.
  • If the species are closely related then the temperature taken to split the two strands is higher.
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