DNA 0.0 / 5 ? Human BiologyDNAUniversityAll boards Created by: molly_axxCreated on: 07-01-19 08:46 What to look at: - 17 loci. - Each loci has 2 short tandem repeats. - Variations in length called alleles. - 32 number string. - Sex markers. 1 of 8 1) EXTRACTION Removal of DNA from source- may need care if sample is old/small. 2 of 8 2) PCR Polymerase Chain Reaction- biological photocopier, makes copies of sample DNA to increase samaple to testing size. 3 of 8 3) VISUALISATION DNA is run through a gel which acts as a biological sieve and separates DNA by size- smaller fragments travel faster than larger ones. 4 of 8 Obtaining the profile - Number from DNA compared to number from reference sample. - Match presented as a probability. - If no match, sample put on NDNAD. 5 of 8 Non-Routine Samples - Very small amounts of DNA can use Low Copy Number (LCN) or Low Template (LT) DNA. - Same process but more PCR cycles. - Mitochondrial DNA for degraded bones and teeth- more stable and lasts longer. 6 of 8 Paternity Testing - Use samples collected from relatives. - mtDNA from Mitochondrial DNA Profiling. 7 of 8 YSTR Profiling - Targets Y chromosomes with STR alleles. - Small amounts of male DNA. 8 of 8
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