Chemical Tests

Gives blue- black colour with iodide solution

• Add 2cm3 of Benedict's reagent to same volume of sample.
• Heat in water bath.
• Brick red shows presence of reducing sugars

• Same as reducing sugars test- showed no colour change.
• Using separate sample, boil with HCl for few minutes.
• Add NaOH to neutralise before adding Benedict's reagent.
• Brick red colour appears

Emulsion Test:

• Shake some sample with 4cm3 of ethanol.
• Decant liquid into water.
• If lipids are present, cloudy solution is formed.

Biuret Test:

• Add 2cm3 of biuret solution to equal volume of sample.
• If protein present, lilac colour will appear.