Cell fractionation is separating organelles of a cell from the rest in order to study them individually.
Key Words before we begin!
Fractionation-the process by which cells are broken up & organelles separated out.
Ultracentrifugigation-process by which fragments in the filtered homogenate are separated in a machine called an ultracentrifuge.
Supernatant-the fluid left over after ultracentrifugigation (contains all organelles that haven't been separated into the sediment)
Isotonic-same concentration of sugars etc as in the organelles
Buffered-means to maintain a constant pH.
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