Dna Hybridisation depends on helical structure of dna
when dna is heated the double strand seperates into 2 complementary strands.these when cooled can rejoin to form a helix once again
Using this property dna hybridisationcan be used to compare dna of species - dna from 2 species is purified and cut into short peices
dna from one of the species is labelled with flouresence or radioactive markers
dna from both of the species is then heated and split it is then left to cool so that the dna can form a helix
some of the strands that join together will be a hybrid of both the species we can then test to see how closely related the species are by heating at different temperature stages
the higher the temp needed to split the strand shows how closely the species are related . this is because there will be more hydrogen bonds between the bases as more complementary pairs are made so more energy needed to break the hydrogen bonds
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