As urea molecules penetrate through the membrane, they bind to the active sites of urase enzymes. The enzyme catalyses the reaction and ammonium ion are produced. The higher the concentration of urea molecules, the greater the number of ammonium ions prouced.The ammonium ions will produce a small current which can be detected by the transducer and a digital reading will be displayed.
Molecules of other solutes which may be present will not affect the reuslt because the partially-permiable membrane will only allow the urea molecules to diffuse through the membrane and so it prevents the passage of other molecules.
The temperature of the biosensor should be kept constant because higher temperature will increase the rate of reaction as molecules will have mole kinetic energy and therefore will collide more frequently. Similarly,the temperature will affect the rate of diffusion
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