Biology A2

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  • Created by: Lucy
  • Created on: 09-09-13 10:35

Using PCR to amplify DNA (To create a DNA profile)

1) A reaction mixture is set up containing the DNA sample, free nucleotides, primers and DNA polymerase.

2) The mixture is heated to 95C to break the hydrogen bonds between the 2 DNA strands

3) The mixture is cooled to 50-65C so that the primers can bind (anneal) to the strands.

4) The reaction mixture is heated to 72C so that the DNA polymerase can work.

5) The DNA polymerase lines up free nucleotides along each template strand, producing new strands of DNA

6) Two new copies of the fragment of DNA are formed and one PCR cycle is complete.

7) The cycle is repeated over and over to produce lots of copies

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