Cells have many mitochondria because they are the site of aerobic respiration. They release energy in the form of ATP. They can also synthesise proteins.
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In ultracentrifugation Cell tissues are homogenised in order to break open the cell and release its contents.
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The resulting suspension is filtered in order to remove cell debris.
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The suspension is kept ice cold because this reduces any potential enzyme activity.
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An isotonic solution is used to prevent osmosis so that organelles are not damaged or burst by excess water enetering the cell.
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