Changing the temperature changes the rate of the reaction
Like with any reaction, a higher temperature increases the rate at first, but if it gets too hot, the protein bonds holding the enzyme together break. This destroys the enzymes special shape, so it wont work anymore. It is said to be denatured.
Enzymes in the human body normally work best at around 37°C (Body Temperature)
The pH also effects enzymes. If its too high or too low, it interferes with the bonds holding the enzyme together. This changes the shape and denatures the enzyme.
All enzymes have an optimum pH that they work best at (beyond this point the enzyme denatures). It is often neutral, at pH 7. But not always. e.g. pepsin, the enzyme used to break down protein in the stomach works best at a very acidic pH 2.
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