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Extracting DNA

1 of 3

Extracting DNA

4) - Cool the mixture by putting the beaker of pea mixture in a water bath of ice for a few minutes 

5) - Ask your teacher to blend the cooled pea mixture for 5 seconds in the blender 

6) - Using a coffee filter paper, filter the pea pieces from the liquid 

7) - Using a siringe put 10cm cubed of the pea solution in a boiling tube and add 2-3 drops of protease enzyme to it 

8) - Measure 10 cm cubed of ice cold ethanol into another boiling tube and very carefully pour it onto the surface of the pea solution 

9) - Bubbles of air carry the DNA and RNA into the upper layer and as DNA doesn't dissolve in ethanol you can see it in the upper layer 

2 of 3

Extracting DNA

4) - Cool the mixture by putting the beaker of pea mixture in a water bath of ice for a few minutes 

5) - Ask your teacher to blend the cooled pea mixture for 5 seconds in the blender 

6) - Using a coffee filter paper, filter the pea pieces from the liquid 

7) - Using a siringe put 10cm cubed of the pea solution in a boiling tube and add 2-3 drops of protease enzyme to it 

8) - Measure 10 cm cubed of ice cold ethanol into another boiling tube and very carefully pour it onto the surface of the pea solution 

9) - Bubbles of air carry the DNA and RNA into the upper layer and as DNA doesn't dissolve in ethanol you can see it in the upper layer 

3 of 3

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