Idea is to put human insulin gene into bacteria so bacteria can make human insulin. Modified bacteria reproduce and you get millions of insulin-producing bacteria.
1. Scientists identify gene controlling production of human insulin.
2. Remove it from DNA of human cell by cutting it out with restriction enzymes leaving DNA with 'sticky-ends'.
3. Loop of bacterial DNA called plasmid then prepared for insulin gene to be inserted. Restriction enzymes used to cut open plasmid, leaving it with 'sticky-ends' too.
4. Insulin gene inserted into plasmid, sticky ends allow ligase enzyme to join DNA strands together. Plasmid taken up by bacteria. Plasmid known as vector, something that carries a gene into another organism.
5. Bacteria now transgenic organism, checked to make sure they contain new gene by assaying techniques and cultured by cloninh. Millions of bacteria can be produced allowing large quantities of insulin to be harvested.
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