Uncontaminated culture: Inoculate = introduce
1. Boil agar, petri dish,inoculated loop, surfaces and agar sterilised.
2. Turn on bunsen burner for convection current.
3. Flame neck of sample bottle, inoculate agar using inoculating loop.(dip in bacteria then zig zagonto gel)
4. Replace lid of petri dish, secure with tape, upside down, stops condensation
5. Incubate 25C, few days, then observe growth
Used for seeing what effects chemicals have (eg antibiotics)
Low temp so it doesn't produce harmful pathogens, only ones that don't reproduce in the body.
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