- When all the tubes have reached 35 °C take one of the tubes of starch from the water bath and add the 2 cm3 of your first pH buffered solution. Stir the mixture with a glass rod.
- Use the pipette to add 2 cm3 of amylase solution to the mixture. Start the stopclock as soon as you add the amylase. Stir with the glass rod.
- After 10 seconds, remove one drop of the mixture with a glass rod.
- Put this drop on the second depression of your spotting tile.
- Every 10 seconds, use the glass rod to remove one drop of the mixture. Put each drop onto the iodine solution in the next depression on the spotting tile. Remember to rinse the glass rod with water after putting each drop on the spotting tile.
- Keep sampling every 10 seconds until the iodine does not change colour.
- Record your results in a table.
Repeat with the other pH buffered solutions.
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