The permeability of cell membranes can be determined by factors such as :
- temperature
- alcohol concentration
There is an experiment that is used to determine cell membrane permeability using beetroot...
- Beetroot cells contain a coloured pigment that leaks out
- The higher the permeability, the more pigment leaks out
How temperature affects beetroot membrane permeability:
(1) Cut 5 equal sized pieces of beetroot and rinse to remove any pigment released during cutting
(2) Place each piece in five different test tubes with 5cm3 of water
(3) Place each test tube in a water bath at a different temperature, e.g. 10 degrees Celsius -----> 50 degrees Celsius
(4) Remove the beetrot from the tubes leaving only the coloured liquid
(5) Use a colorimeter - a machine that passes light through the liquid and measures how much of that light is absorbed.
-The higher the absorbance -----> more pigment released -----> higher membrane permeability
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