Test for Redcing Sugars:
1) Add Benedicts reagent (which is Blue) to a sample and heat it in a waterbath until it boils.
2) If the test is positive it will form a coloured precipitate (solid particles in the solution/sample).
3) The higher the concentration of the reducing sugar the more the colour will change, this allows you to compare the amount reducing sugar in the sample. Then you can weigh the percipitate to get accurate results.
Colour of Precipitate: Blue -> Green -> Yellow -> Orange -> Red
If the results of the reducing sugar test are negative, non reducing sugar caould be present. To check, the sugar needs to be broken down down into monosaccharides.
1)Get a new sample of test solution, adding dilute hydrocholoric acid and heat it in the waterbath until it boils. Then neutralise it with sodium hydrogencarbonate.
2) Continue Benedicts test as normal and record the results (colour and positive/negative)
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