trude

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  • Created by: lenny
  • Created on: 18-11-12 12:54
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Lecture 11: Biotechnology
Crown gall tissue forms
only at wound sites
Wound response - limited
activation of cell division
[secretion of phenolic
compounds]
· Grows even after
antibiotic treatment
to kill the
Agrobacterium
· Bacteria do not need
to be continuously present to cause their effect
· Crown gall cells are transformed

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TRANSFORMED CELLS HAVE NEW PROPERTIES
Transformed tissue Proliferative tissue without transformation
Grow in the absence of exogenous hormones Require both auxin and cytokinins
Contain high levels of auxins and cytokinins Contain low levels of endogenous auxins and
cytokinins
Synthesize opines Do not synthesize opines
Transgenic plants
Plant cells are totipotent…read more

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Cloned genes can be introduced into somatic tissue and entire plant regenerated with
hormonal treatments
Agrobacterium tumefaciens naturally infects plant cells and causes tumors (T37 in practicals)
o Contains Ti plasmid that integrates into host chromosome
o Codes for plant growth hormones that form crown gall tumor
Ti plasmid modified to introduce cloned genes
o KanR used as a selectable marker for kanamycin resistance
o Contain convenient restriction sites
Transformed cells plated on media with kanamycin (kills nontransformed cells) and
carbenicillin (kills Agrobacterium)
Plant Genetic…read more

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