Slides in this set

Slide 1

Preview of page 1

Slide 2

Preview of page 2

What is Chromatography?
Chromatography is a technique for
separating mixtures into their components
in order to analyze, identify, purify,
and/or quantify the mixture or
· Analyze
Separate · Identify
· Purify
· Quantify
Mixture Components…read more

Slide 3

Preview of page 3

Uses for Chromatography
Chromatography is used by scientists to:
· Analyze ­ examine a mixture, its components, and
their relations to one another
· Identify ­ determine the identity of a mixture or
components based on known components
· Purify ­ separate components in order to isolate
one of interest for further study
· Quantify ­ determine the amount of the a mixture
and/or the components present in the sample…read more

Slide 4

Preview of page 4

Uses for Chromatography
Real-life examples of uses for
· Pharmaceutical Company ­ determine amount of
each chemical found in new product
· Hospital ­ detect blood or alcohol levels in a
patient's blood stream
· Law Enforcement ­ to compare a sample found at
a crime scene to samples from suspects
· Environmental Agency ­ determine the level of
pollutants in the water supply
· Manufacturing Plant ­ to purify a chemical
needed to make a product…read more

Slide 5

Preview of page 5

Definition of Chromatography
Detailed Definition:
Chromatography is a laboratory technique that
separates components within a mixture by using the
differential affinities of the components for a mobile medium
and for a stationary adsorbing medium through which they
· Differential ­ showing a difference, distinctive
· Affinity ­ natural attraction or force between things
· Mobile Medium ­ gas or liquid that carries the components
(mobile phase)
· Stationary Medium ­ the part of the apparatus that does
not move with the sample (stationary phase)…read more

Slide 6

Preview of page 6

Definition of Chromatography
Simplified Definition:
Chromatography separates the components of a
mixture by their distinctive attraction to the mobile
phase and the stationary phase.
· Compound is placed on stationary phase
· Mobile phase passes through the stationary phase
· Mobile phase solubilizes the components
· Mobile phase carries the individual components a
certain distance through the stationary phase,
depending on their attraction to both of the
phases…read more

Slide 7

Preview of page 7
Preview of page 7

Slide 8

Preview of page 8

Slide 9

Preview of page 9
Preview of page 9

Slide 10

Preview of page 10
Preview of page 10


No comments have yet been made

Similar Chemistry resources:

See all Chemistry resources »