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Module 1: Biological Molecules
2.1.3 Enzymes
(a) state that enzymes are globular proteins, with a specific tertiary structure, which catalyse metabolic
reactions in living organisms
All enzymes are globular proteins with a specific tertiary
structure, which catalyse metabolic reactions in all living
organisms. This means that they speed up chemical…

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Module 1: Biological Molecules
(d) describe and explain the effects of pH, temperature, enzyme concentration and substrate concentration on
enzyme activity
Effect Rate of Reaction Graph
pH At different pH's, the hydrogen ions interfere with
the hydrogen bonds and ionic bonds holding the
tertiary structure in place. Therefore the shape…

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Module 1: Biological Molecules

(e) describe how the effects of pH, temperature, enzyme concentration and substrate concentration on enzyme
activity can be investigated experimentally
Variable Method of Keeping Constant Reasons
pH Use buffer solutions that maintain pH at Reaction rate depends on the pH as if it is not at…

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Module 1: Biological Molecules
more likely than an inhibitor to collide with an active
site.















Reversible inhibitors are inhibitors that bind to the active site for a short period and then leave. The removal of the
inhibitor from the reacting mixture leaves the enzyme molecules unaffected.

Irreversible inhibitors are inhibitors…

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Module 1: Biological Molecules




(h) state that metabolic poisons may be enzyme inhibitors, and describe the action of one named poison
Potassium Potassium cyanide inhibits cell respiration.
Cyanide It is a noncompetitive inhibitor for a vital respiratory enzyme called cytochrome
oxidase, found in mitochondria.
Inhibition of this enzyme decreases the…

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