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Growth, Detection and Characterization

Gram negative bacteria do not retain crystal violet dye in the gram staining protocol. Gram positive
bacteria stain dark blue or violet by gram staining. Classification is based on the structural differences
of their cell walls: Gram positive have thick peptidoglycan layer.

Binary fission is asexual…

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Special Media ­ Based on oxygen requirement, aerobic/anaerobic media. A simple way is a
stab culture. Anaerobic jar is used to culture anaerobic bacteria, it contains sodium
bicarbonate and sodium borohydride.
Liquid Media ­ No agar. Used for inoculum preparation for blood samples, or isolation of
pathogens. E.g Nutrient broth…


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