Experiments based on photosynthesis

Includes :

  • Testing for starch
  • Demonstrating that light is needed for photosynthesis
  • Demonstrating that chlorophyll is needed for photosynthesis
  • Demonstrating that Carbon Dioxide is neede for photosynthesis
  • Is oxygen a result of photosynthesis?
  • The effect of carbon dioxide concentration on the rate of photsynthesis
  • The effect of light intensity on photosynthesis
  • The effect of temperature on photosynthesis
  • The effect of nitrogen deficiency on a healthy plant
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  • Created by: Rachel
  • Created on: 13-05-11 13:22
Preview of Experiments based on photosynthesis

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Experiments for Photosynthesis
Biology
1) Testing for Starch
Destarch a plant by placing it in a dark room for 48 hours
Cover some leaves with aluminium foil
Place the plant in the light for 24 hours
Boil water in two separate water baths using a Bunsen burner
Cut one covered and one uncovered leaf
Place each in separate boiling tubes which contain water
Place each tube in a separate water bath
The leaves are removed and blotted dry with filter paper
The water in the boiling tubes are replaced by ethanol
Insert the leaves back into the tubes
The leaves are removed and blotted dry with filter paper
Each leaf is placed in separate Petri Dishes
A few drops of Iodine solution are added and the colour changes are recorded
2) Demonstrating that light is needed in photosynthesis
Take two plants of the same size and species
Destarch both for 48 hours
Keep one plant in the dark room
Place the other plant in light
Cut a leaf from each plant
Place them in separate Petri Dishes
Add a few drops of iodine to test for starch
Record the colour changes
3) Demonstrating that chlorophyll is needed in photosynthesis
Destarch a variegated plant for 48 hours
Place it in the light for 24 hours
Cut a leaf and place it in a Petri Dish
Add a few drops of iodine
Record the colour changes
(The white part of the plant with remain brown as there is no chlorophyll but the
green part of the leaf will turn from brown to blueblack )
4) Demonstrating that Carbon Dioxide (CO) is needed in photosynthesis
Destarch two plants for 48 hours
A) Then one is place in a container with a filter pump (to remove unwanted gases)
and an open tube which has a stopper attached to it
The plant's base is covered with a polythene bag to stop water vapour from
escaping
Next to the plant in the container is Sodium Hydrogencarbonate which produces
carbon dioxide

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This set up is left in the light for 24 hours
(Results: The plant was tested positively for starch )
B) The other plant was placed in another container with a filter pump and a tube
bringing in sodalime attached to the stopper in the opening
The plant's base was covered with polythene
Next to the plant in the container is Potassium Hydroxide
This setup is left in the light for 24 hours
(Results: Since NaOH and KOH absorb carbon dioxide, there was no CO…read more

Page 3

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The set up is placed on a retort stand
A Bunsen burner is placed under the stand
This set up is repeated five times with the water being at different temperatures
(Results: At a low temperature a small amount of oxygen was produced but as
the temperature increased so did the amount of O produced. Beyond a certain
temperature i.e. the optimum temperature, the rate decreases since the enzymes
denatured.…read more

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