Daphnia Practical

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anaH
Daphnia Heart Rate
:Aim
To investigate the effect of caffeineon the heart rate of Daphnia
:Background
Plants produce caffeine as an insecticide. Caffeine has medicinal uses in aspirin
preparations and weight-loss drugs. In humans, caffeine has been linked to
restlessness, insomnia and anxiety, causing raised stress and blood pressure. This
.can lead to heart and circulatory problems
:Hypothesis
I think that the amount of caffeine in the blood will determine the heart rate of the
daphnia. It will be directly proportional to each other i.e. an increase in % of caffeine
.will increase the heart rate of the daphnia
:Equipment
Culture of Daphnia
Cavity slides
dropping pipettes
Distilled/pond water
Caffeine solutions
Cotton wool
Stopwatch
Microscope
Paper towels/filter paper
1

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:Method
Place a few strands of cotton wool on a cavity (this restricts the movement of the .1
.(water flea
.Transfer one water flea to a cavity slide with a pipette .2
.Remove any water with filter paper and add distilled water or pond water .3
.(Place a cavity slide under the slide (acts as a heat sink .4
.View under a microscope under low power .…read more

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H
:Results
.Experiment 1 ­ Daphnia were put on ice 30 minutes before the experiment
Treatmen Mean for individual/ beats
t Heart rate / beats 30 s-1 min -1
Trial 1 Trial 2 Trial 3 Mean
Caffeine
Caffeine
Caffeine
Caffeine
Caffeine
Control
Control
Control
Control
Control
.…read more

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