chromatography revision

HideShow resource information
  • Created by: Anonymous
  • Created on: 08-10-07 09:32
Preview of Chromatography

First 126 words of the document:

This technique separates out mixtures of chemicals by using their different solubilities in
certain solvents. A concentrated spot of the solution to be separated is placed at one
corner of a strip of paper. This is dipped in a solvent which carries the dissolved chemicals
up the paper depositing them, the least soluble first, the most soluble last.. I f necessary,
other chemicals may be used to make the spots visible e.g. Ninhydrin colours amino acids.
They are identified by their colour and Rf values:
Rf = Distance moved by spot
Distance moved by solvent
Spots may be further separated by running a different solvent at right angles to the first
(two-way chromatography).


No comments have yet been made

Similar Biology resources:

See all Biology resources »See all resources »