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Unit 2.1.1. OCR

Biochemical Tests For Molecules

Benedict's Test for Sugars
Sugar ­ Monosaccharides + disaccharides.
Classified as reducing or non-reducing.
Test for sugars ­ Benedict's Test

All Monosaccharides (e.g. glucose) + some disaccharides (e.g.
1. Add Benedict's reagent (blue) to a sample.
2. Heat ­ but…

Page 2

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Unit 2.1.1. OCR

2. Add copper(II) sulfate solution.
Protein Present ­ purple layer forms.
No Protein ­ Stays blue

Emulsion Test for Lipids
1. Add ethanol.
2. Shake.
3. Pour into water.
Lipid present ­ turns milky ­ the more lipid, the more noticeable.
No lipid ­ solution stays clear.…


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