AS EDEXCEL BIOLOGY CORE PRACTICAL UNIT 2

Basically, as the title suggests Unit 2 (Topic 3 and 4) Edexcel core practicals!

Any questions message me, I don't bite :)

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  • Created by: Parm
  • Created on: 21-05-13 19:27
Preview of AS EDEXCEL BIOLOGY CORE PRACTICAL UNIT 2

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Plant mineral deficiency Antibacterial properties of plants
Independent Variable: Concentration of the Mineral Independent Variable: The presence of either Garlic or Mint
Dependent Variable: The effect of mineral deficiency on a plant Dependent Variable: Area of the zone of inhibition of the Garlic and the Mint
Control: The same plant, temperature and light exposure Control: Concentration of the plant material, amount of bacteria in the petri dish, the same volume of plant
solution on each disk
Equipment:
Equipment:
Test tubes prepared with different concentration minerals Paper discs
Plant 3x Test tubes
Aluminium foil Mortar and pestle
Petri dish with prepared Agar solution
Risks and how to minimise them: Mint
Be careful when making the hole with a scalpel ­ handle the scalpel with care. Garlic
Handle the test tubes with care in case it breaks ­ make sure you hold the test tube tightly and Forceps
place in a test tube rack to make sure they don't roll off. Cellotape
Methylated spirit
Method:
1. Get three test tubes with different concentrations of minerals. Risks and how to minimise them:
2. Cover the three test tubes with aluminium foil so any other factors are not The methylated spirit is toxic and highly flammable ­ make sure there is no naked flame in use whilst
influenced even on the top ­ making a small hole with a scalpel. carrying out the experiment
3. Carefully place the plant in the hole. Handle the test tubes with care in case it breaks ­ make sure you hold the test tube tightly and place in a
4. Place on a windowsill where the sun is able to reach it and observe it constantly. test tube rack to make sure they don't roll off.
NOTE: You can also carry out this practical with different types of minerals too.
Method:
Observation: The plant with the most concentration would grow the most and the one with the 1. Collect a Petri dish with prepared Agar solution
least will grow the least. 2. Place 3g of mint into a pestle and mortar along with 10cm3 methylated spirit.
3. Do the same for the garlic.
Evaluation methods: 4. Place the substance into a test tube and shake for 10 minutes.
5. Dip two paper disks into the different plant solutions holding them with forceps.
May grow some unwanted pathogens in the Agar minerals solution
6. Pick up the paper disks using the forceps and place them into the Agar flat and carefully.
Time restraints
7. Follow aseptic techniques and place tape Cellotape
8. Label the Petri dish with what substance is what.
Observation: The paper disk with the mint will have the biggest area of zone of inhibition, meaning that the
most bacteria were killed.
Evaluation methods:
May grow some unwanted pathogens in the Agar solution because aseptic technique were not carried
out properly.
Not shaking the substance well.
Not a control amount of plant mineral added.
Resource obtained from:
Resource obtained from:
http://getrevising.co.uk/members/p_choudhry
http://getrevising.co.uk/members/p_choudhry

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Tensile strength of plant fibres:
Observing Totipotency:
Independent Variable: The weights added to the plant fibre
Independent Variable: N/A
Dependent Variable: The mass that a plant fibre can take
Dependent Variable: The affect on Agar nutrients when a plant is placed in
Control: The same Agar nutrients, amount of Agar nutrients, temperature and light exposure.…read more

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Root tip Squash:
Independent Variable: N/A
Dependent Variable: N/A
Control: N/A
Equipment:
Root tip
Glass container
Hydrochloric acid
Acetic orcein (stain)
Slide
Scalpel
Cover slip
Microscope
Risks and how to minimise them:
The stain may go onto your hands ­ wash it off immediately.
Method:
1. Cut off 5mm off a root tip from an onion.
2. Place the tip into a glass container and add 2-3 drops of hydrochloric acid to soften
and break the membrane
3.…read more

Comments

Hafsa


cool!! :)

Glorious

Thanks

Lana

Hey it's Lana! These are really good! If only I found them before my exam *sigh* LOL.

Parm

Thanks Hafsa and Glorious, and haha, sorry Lana, I'm sure you don't need them anyway! You're so clever :p

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