A GRADE catalase Coursework

To investigate the effect of temperature on the rate of reaction of an enzyme “catalase” by measuring the amount of oxygen produced by the reaction. I am going to investigate the effect that temperature has because this is an easy variable to change and control using water baths. Catalase is found in all cells and catalyses the reaction.

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  • Created on: 13-01-11 12:29
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George O'Keefe GCSE Biology
Aim
To investigate the effect of temperature on the rate of reaction of an enzyme "catalase" by
measuring the amount of oxygen produced by the reaction. I am going to investigate the effect
that temperature has because this is an easy variable to change and control using water baths.
Catalase is found in all cells and catalyses the reaction.
Hydrogen Peroxide Water + Oxygen (H2O2 H2O + O2)
Preliminary Experiment Results
Yeast (g) O2cm³ / 30 seconds
0.1 28
0.2 32
0.3 35
0.4 34
0.5 40
From my preliminary experiment I discovered that the increased amount of yeast you use, the
increased amount of oxygen gas is produced. For my final experiment I will use 0.3g of yeast
because I want an amount that will perform to it's optimum capacity in order to gain reliable
data. The reason why I am not using 0.4g or above is because in my preliminary experiment it
created a more violent reaction causing the yeast to rise and it clogged up the delivery tube
which restricted the gas flow consequently it could affect the final result.
Rate of an enzyme catalased chemical reaction can be affected by the following variables`:
Temperature (controlled in our case)
pH level
The concentration of reactants (hydrogen peroxide, yeast and catalase)
Method
I will measure 5cm³ of Hydrogen Peroxide using a 10cm³ measuring cylinder in order to gain
an accurate measurement, I will pour the substance carefully into a clean test tube. Then I will
place it in a water bath until it reaches 20C. Then using a measuring balance I will measure
0.3g of yeast and place it into a boiling tube. I will use an accurate balance so I can gain a
precise amount of yeast. I will then add the Hydrogen peroxide to the yeast and connect it to
the delivery tube. I will then using an accurate timing device in order to gain the precise time
of the reaction. After 30 second I will record the amount of Oxygen produced and I will
repeat this 3 times to make my results more reliable. I will also repeat the same procedure but
increase the temperature by 10C each time. I will do this until I have got three set of results
for each temperature until I reach 70C. I will wear safety glasses to protect my eyes

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George O'Keefe GCSE Biology
and all my equipment will be clean along with my hands in order to keep the area sterile to
prevent foreign substances hindering the reliability of my results.…read more

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George O'Keefe GCSE Biology
30 44
40 33.5
50 50.6
60 62.5
70 29
Conclusion
From the graphs I have drawn I can see sings of some relationships in the data. There is a
positive correlation in the results except for the outliers. The amount of Oxygen increases to
rise until 60C other than the results taken at 40C, indicating their was a an error in my method
whilst conducting the experiment at 40C.…read more

Comments

jon zobee


doesn't look like A grade

 

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