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Emily Summers

Artificial DNA replication, carried out on small samples of DNA to create many
copies of the sample, useful in forensics. This is called amplification.

Sequencing reaction relies on:
The DNA is made up of antiparallel backbone strands
Made up of strands with a 5' prime and a 3'…

Page 2

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Emily Summers

Start with a sample of the DNA to be amplified, and add the four nucleotides and
DNA polymerase.

Separate strands by heating to 95°C for two minutes. This breaks the hydrogen

DNA polymerisation always requires primers which are separately synthesised
and added at this stage. This means…


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