Immobilised enzymes - Advantages and disadvantages table in A Level and IB Biology

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Created by: emilyrose5
Created on: 09-05-16 10:49

Immobalised enzymes

Advantages

Once column set up can be repeatably used
products not contaminated with enzyme
increases stability and function over a wider range (higher temps, bigger changes in pH)
enzymes easly recovered for reuse
enzymes easily added or taken out
can use a sequence of columns to allow use of several different enzymes in different conditions
trapping the enzyme prevents the shape change

Disadvantages

Enzymes immobilised on needs have a lower rate of reaction as some enzymes on the inside of needs so can take longer for substrate to diffuse in
costs a lot of money to set up

Evaluation

Enzymes are immobilised when they are fixed, bound or trapped in a inert matrix - E.g on sodium aligent beeds or cellulose microfibrils - they are used in many industrial processes

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