Immobilised enzymes


Immobalised enzymes


  • Once column set up can be repeatably used
  • products not contaminated with enzyme
  • increases stability and function over a wider range (higher temps, bigger changes in pH)
  • enzymes easly recovered for reuse
  • enzymes easily added or taken out
  • can use a sequence of columns to allow use of several different enzymes in different conditions
  • trapping the enzyme prevents the shape change


  • Enzymes immobilised on needs have a lower rate of reaction as some enzymes on the inside of needs so can take longer for substrate to diffuse in
  • costs a lot of money to set up


Enzymes are immobilised when they are fixed, bound or trapped in a inert matrix - E.g on sodium aligent beeds or cellulose microfibrils - they are used in many industrial processes


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