The effect of PH on the activity of cellulase

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The question for the bilogy AS evaluative test ......
1- when doing an average why should you not take out anomalous results
2- what happens when you increase PH
3- what would you do if you set the experiment for effect of temperature on activity of cellulase and what would be the control and buffers
4- why is it difficult to observe or predict the PH

Posted Wed 18th April, 2012 @ 18:17 by M.Ahmed

3 Answers

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1.) i think this question is probably worded wrong because when you calculate the mean, any result that looks like an anomaly that doesn't follow the trend is left out when calcualting the average. If you don't then your results are not accurate at all because you are using anomalous results to calculate a mean!

2.) This question depends on the experiment you did. If the optimum pH for cellulase (did this experiment but cant remember esults soz..) is acidic then if you increase the pH then the rate of reaction will decrease significantly as you enter the alkaline region as it changes the balance of OH- and H+ ions in the solution which affects the enzymes structure making it become denatured therefore less collisions, less E-S complexes formed.

3.)Buffers would most likely be a acidic pH at around 1-2-3. Controls include pH, volumes used, time left in solution etc.

4.) It is difficult to observe the pH because to actually measure it can be quite difficult for exmaple using universal indicator is not accurate at all and even litmus paper only tells you whether it is acid/alkaline but not a precise pH number. Also the pH may change continuosly if a buffer is not used during the experiment therefore making it hard to predict.

I think these could be part of the answer. ike i mentioned i think the first question might have been typed wrong or something because they wouldn't ask a question like that (well i hope so!) but hope i helped and like Albert Einstein said......

"I allow my work to be criticised. If my theories are proven wrong then i shall be happy" (or something like that)

Anyway hope i helped, and if any further explanation is needed just comment again and ill be glad to explain in another way =)

Answered Wed 18th April, 2012 @ 21:12 by Braniac
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What exactly is a buffer

How would you set up an experiment for measuring effect of temprature on the enzyme activity if catalase

Answered Thu 19th April, 2012 @ 18:14 by M.Ahmed
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have you had this evaluative already? what other questions were ther?

Answered Thu 19th April, 2012 @ 20:25 by skh