How does the temperature and pH affect the activity of amylase?

  • 0 votes

Im not sure, i know the enzyme will get denatured but i need more information...

Posted Sun 9th September, 2012 @ 12:12 by S.H
Edited by S.H on Sun 9th September, 2012 @ 12:12

5 Answers

  • 2 votes

Enzymes and temperature

At low temperatures, enzyme reactions are slow. They speed up as the temperature rises until an optimum temperature is reached. After this point the reaction will slow down and eventually stop.

Temperature and enzyme activity (http://www.bbc.co.uk/schools/gcsebitesize/science/images/add_edex_bio_enzyme-chart.jpg)

Enzymes and pH

Most enzymes work fastest in neutral conditions. Making the solution more acidic or alkaline will slow the reaction down. At extremes of pH the reaction will stop altogether. Some enzymes, such as those used in digestion, are adapted to work faster in unusual pH conditions and may have an optimum pH of 2 (very acidic) if they act in the stomach.

pH and enzyme activity (http://www.bbc.co.uk/schools/gcsebitesize/science/images/add_edex_bio_ph-chart.jpg)

Answered Sun 9th September, 2012 @ 12:20 by Lullu
  • 2 votes

Hope that helped a little!!!! :)

Answered Sun 9th September, 2012 @ 12:21 by Lullu
  • 0 votes

Thankyou!! It helped a lot!! :D

Answered Sun 9th September, 2012 @ 12:27 by S.H
  • 0 votes

You're welcome! ;)

Answered Wed 12th September, 2012 @ 17:53 by Lullu
  • 0 votes

Exactly what Lullu has said and

if they are denatures enzyme substrate complex cannot be formed as the active site is no longer a complementary shape because the tertiary structure of the amino acids has been disrupted, which changes the shape of the protein

That is a A level answer, might be a bit advanced if you are doing GCSE 

Answered Fri 14th September, 2012 @ 00:02 by Alex