{ BIO } OCR F212 2.1.1 Tests

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  • Created by: kaen
  • Created on: 29-05-15 13:33
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  • TESTS
    • PROTEIN
      • biuret test
        • dilute sodium hydroxide
        • dilute copper sulfate
          • purple = positive
            • dilute sodium hydroxide
    • REDUCING SUGARS
      • add Benedict's solution
        • heat in water bath
          • blue - orange precipitate
    • NON REDUCING SUGARS
      • boil with HCL
        • neutralise with sodium hydrogen-carbonate
          • Benedict's test
    • STARCH
      • iodine solution
        • yellow - blue/black
    • LIPIDS
      • emulsion
        • add alcohol
          • shake and settle
            • add to water in test tube
              • cloudiness = lipid
    • COLOROMITER
      • filter out precipitate
        • zero device with blank curette
          • take readings from known conc.
            • create calibration curve
              • measure % trans-mission of unknowm
                • compare on curve

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