How to test a leaf for starch
- Created by: Katy **
- Created on: 31-12-13 09:31
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- Test leaf for starch
- Step 1.
- 'destarch' plant
- place in dark
- 24-48 hours
- Step 2.
- place leaf in boiling water
- 5 mins
- destroys cell wall & membrane
- this will let ethanol & iodine in & chlorophyll out of the leaf
- place leaf in boiling water
- Step 5.
- few drop of orange iodine
- leaf turns blue-black
- starch present
- leaf turns blue-black
- few drop of orange iodine
- Step 3.
- put leaf in warm ethanol
- ethanol dissolves fat solvent molecules (chlorophyll)
- put leaf in warm ethanol
- Step 4.
- extract all chlorophyll ? leaf turned white
- easier to see iodine colour change
- extract all chlorophyll ? leaf turned white
- Safety
- turn off budsen burners
- ethanol = flammable
- turn off budsen burners
- Step 1.
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