Synergy
- Created by: natasha8sherry
- Created on: 10-11-13 15:52
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- Synergy
- 2+ activators acting together - MORE than additive activation
- Multiple TAFs and synergistic activation experiment
- Hunchback (HB) and Bicoid (BCD) only activators required in vivo for transcription of promoter controlling D. melanogaster gene hunchback (hb)
- Partial TFIID complexes used for in vivo transcription: 1) TBP, TAF1, TAF6 2) TBP, TAF1, TAF4, 3) TBP, TAF1, TAF 4 + TAF4
- During experiment DNA, RNAP II and 3 partial complexes added - see how much radiolabelled RNA produced
- BCD requires TAF 4
- HB requires TAF 6
- Bicoid helps pull TFIID to promoter by binding TAF4 and HB protein pulls TFIID by binding with TAF 6
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