Substrate - level posphorylation- glycolysis - link reaction - kreb's cycle

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  • Substrate - level posphorylation- glycolysis - link reaction - kreb's cycle
    • Substrate-level phosphorylation
      • 1) Glucose is phosporylated (phosphate comes from ATP)
      • 2) The sixth carbon - hexose phospahte - splits into two (lysis splitting of 3C triose phosphate molecules)
      • 3) The 3C triose phosphates are dehydrogenated/oxidised and converted into pyruvate
      • 4) Energy from the triose phosphate bonds is used to combine ADP + Pi ---> ATP
      • Energy budget for glycolysis
        • 4 ATPs have been made but 2 have been used, so NET ATP = 2
        • 2 Reduced NAD due to reaction
        • 2 Pyruvates
      • Takes place in the cytoplasm of the cell and does not require oxygen
    • Link Reaction
      • Occurs in the matrix of the mitochondria and diffuses in as pyruvic acid
      • 1) Pyruvate is decarboxylated (removal of CO2) and dehydrogenated (removal of H), hydrogen is picked up/accepted by NAD ---> reduced NAD
      • 2) This results in acetate (2C) and this joins with Co enzyme A (2C)
      • Energy budget for link reaction
        • 2 acetyl co enzyme A (acetyl CoA) goes into the Krebs Cycle
        • 2CO2 (is released as waste products)
        • 2 Reduced NAD (go to the electron transport chain)
    • Krebs Cycle
      • Occurs in the matrix
      • Energy budget for the Krebs cycle
        • 2CO2
        • 6NADP
        • 2 FADP
        • 2ATP


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