RESPIRATION

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  • RESPIRATION
    • ELECTRON TRANSPORT CHAIN (ETC)
      • oxidative phosphorylation
      • cyanide is an oxygen inhibitor (die)
      • Electron Transfer Chain
    • MEASURING RESPIRATION
      • usually use yeast cells
        • respire anaerobically & aerobically & multiply quickly
      • measure oxygen uptake
        • vol. = pie r2 x distance water moved
      • Measure carbon dioxide output
        • pH indicator (carbonic acid is acidic)
      • dehydrogenase activity
        • DCPIP turns colourless
        • methylene blue turns colourless
    • LINK REACTION
      • occurs on inner mitochondrial membrane to matrix
      • carbon dioxide & lipid will diffuse out
      • The Link Reaction
    • PROTEINS
      • only when starved
      • protein is converted into amino acids
        • amino acids form pyruvate & acetyl co.A
    • ANAEROBIC CONDITIONS
      • only 2 ATP molecules are made
      • in animals & bacteria
        • lactic acid produced
      • in plants
        • ethanoic acid & carbon dioxide produced (plant will die)
    • KREBS CYCLE
      • occurs in mitochondrial matrix
      • oxidative phophorylation
      • The Krebs Cycle
    • LIPIDS
      • energy store when sugar levels are low
      • fat converted into fatty acids or glycerol
        • glycerol forms TP
        • fatty acids forms acetyl co.A
      • twice the amount of energy is made
    • GLYCOLYSIS
      • occurs in cytoplasm (cell membrane to outer mitochondrial membrane)
      • Glycolysis
      • substrate level phophorylation
      • insulin is the glucose carrier (moves by passive transport)

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