RECOMBINANT DNA
- Created by: coffeecraver
- Created on: 29-03-17 15:02
View mindmap
- Recombinant DNA
- Restriction enzymes
- Locate specific palindromic sequences
- Sequences that read the same forward and backward
- To which the restriction binds and cleaves
- Sequences that read the same forward and backward
- Produces sticky ends
- Locate specific palindromic sequences
- Transgenic
- Organisms containing foreign DNA
- Transgene
- The introduced foreign gene
- The extract and cut up DNA from donor genome into fragments containing the desired gene using the same restriction enzyme
- Use the same restriction enzyme used to cut the bacterial or virular plasmid to create complement sticky ends
- Although restriction enzymes were used, different cells may have taken up the DNA in different ways or not at all
- For this reason, electrophoresis is used to determine which cells contain the desired gene and which cells to reproduce
- Although restriction enzymes were used, different cells may have taken up the DNA in different ways or not at all
- Gene fragments will insert into the vector
- DNA ligase seals the fragment and vector together
- Use the same restriction enzyme used to cut the bacterial or virular plasmid to create complement sticky ends
- Ethics
- Somatic therapy
- Changes/ fixes/ replaces genes in just one person. The targeted cells are only the ones affected, the changes are not passed on to that persons offspring
- Germ line therapy
- DNA is transferred into the cells that produce reproductive cells. This type of therapy allows for the correction of disease causing gene variants that are certain to be [passed down from generation to generation
- Somatic therapy
- Antibiotic
- Added recombinant DNA that codes for antibiotic resistance so that the actual gene you want isn't disregarded as the organism doesn't need it.
- You give them something they do need so that they keep the small bit of DNA you want them to keep and create stuff with
- Added recombinant DNA that codes for antibiotic resistance so that the actual gene you want isn't disregarded as the organism doesn't need it.
- Also called chimeric DNA
- After the new DNA is taken up, cells change and processes differ
- Increased production of hormones
- Secretion of new products
- Bacterial cells asexually reproduce
- The recombinant DNA is passed on through genetic material to future generations
- After the new DNA is taken up, cells change and processes differ
- Uses
- Genetic engineering
- The use of recombinant DNA to alter the genotype and phenotype of another organism
- Make proteins
- Read DNA sequence and predict protein sequence
- Analysis of mRNA structure and expression
- Analysis of genomic DNA
- Genetic engineering
- Restriction enzymes
Comments
No comments have yet been made