Proteomics
- Created by: christinamac1
- Created on: 14-01-18 11:38
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- Proteomics
- Proteomics - study of proteome and proteins.
- Proteomics more complex than genomics because the genome is relatively constant whereas the proteins found in one cell type will differ from another cell type.
- DNA sequencing allows the genome to be analysed.
- Transcriptome - collection of all RNA molecules produced by a genome.
- Includes mRNA, tRNA, rRNA.
- Microarray analysis - analysis of the transcriptome
- 1. mRNA extracted from cell
- 2,Reverse Transcriptase enzyme used to convert mRNA into DNA copy - cDNA
- 3. cDNA labelled with a fluorescent probe,
- 4.cDNA added to a microarray which contains DNA sequences.
- 5. Laser used to identify if cDNA has bound to any complementay DNA fragments.
- 6. Allows scientists determine which genes are expressed in different cell types.
- 5. Laser used to identify if cDNA has bound to any complementay DNA fragments.
- 4.cDNA added to a microarray which contains DNA sequences.
- 3. cDNA labelled with a fluorescent probe,
- 2,Reverse Transcriptase enzyme used to convert mRNA into DNA copy - cDNA
- 1. mRNA extracted from cell
- Transcriptome - collection of all RNA molecules produced by a genome.
- Proteomics - study of proteome and proteins.
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