Movement of Substances across Membranes

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  • Created by: Jacqui2
  • Created on: 18-05-17 14:14
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  • Movement of Substances across Membranes
    • Diffusion
      • Diffusion is the free passage of molecules (and atoms and ions) from a region of high to low concentration
      • The energy comes from the kinetic energy of molecules
      • Diffusion in Cells occurs where:
        • The plasma membrane is fully permeable to the solute
        • Water diffuses across the plasma membrane via membrane proteins called aquaprions, and tiny spaces
      • Facilitated Diffusion
        • Allows faster movement with the use of proteins to assist or facilitate diffusion
        • Channel Protein
          • Open spaces and pores across the membrane
          • Usually specific for one ion
        • Carrier Protein
          • Allow larger polar molecules such as sugars and amino acids to diffuse across
          • Molecule attaches to the carrier protein at its particular binding site
            • Changes shape
      • Osmosis
        • Diffusion of water molecules from a region of high water potential to a region of low water potential through a partially permeable membrane
        • Water Potential = Solute Potential + Pressure Potential
          • Water Potential
            • A weak solution will have a high conc of water molecules - lots of free water molecules
              • Free water molecules with lots of kinetic energy
            • Pure water has the highest water potential at 0 (water pot at all solutions will be negative)
          • Solute Potential
            • Conc of dissolved substances
            • Value is always negative - solute molecules reduce the no. of free water molecules
            • A measure of the reduction in water potential due to the presence of solute molecules
          • Pressure Potential
            • The pressure of the cell wall develops as water goes into it
              • Cell is said to be turgid when no more water can enter the cell - hypotonic solution
            • Value is positive
            • The pressure exerted on the cell contents by the cell wall and cell membrane
            • Only present in plant cells due to the presence of a cell wall
        • Plasmolysis
          • When the water potential is higher in the plant cell than an external solution - hypertonic solution
          • The cell surface membrane starts to shrink away from the cell wall as water leaves the cell
            • When pressure pot. is zero the cell is flaccid (point of incipient plasmolysis
              • When 50% of cells are plasmolysed it is said to be at incipient plasmolysis
          • As more water leaves the cell the membrane becomes completely detached and the cell is plasmolysed
    • Active Transport
      • The uptake of molecules or ions against a concentration gradient using energy from respiration
        • Respiration rate can be affected by:(controlled by enzymes)
          • Temperature
          • Oxygen conc
          • Presence of poisons
      • Relies upon specific carrier proteins - needs an input of energy, provided by ATP
        • Molecule or ion binds with a specific carrier protein (binding site)
          • Undergoes a change in shape which carries molecule to the other side of the membrane
            • Molecules or ion is released and protein reverts to its original shape
      • Processes that involve active transport
        • Nerve impulse transmission
        • Muscle contraction
        • Absorption of mineral salts by plat roots (transpiration)
        • Excretion of urea by the kidney
    • Endocytosis and Exocytosis
      • The cell can transport large quantities of material into or out of the cell
      • Endocytosis
        • Passage of material into the cell
          • Phagocytosis
            • Solid material
            • Lysosomes fuse with vesicle
            • Enzymes digest the material and the products are absorbed into the cytoplasm
            • Phagocytes remove bacteria and cell debris by phagocytosis
          • Pinocytosis
            • Liquid material
            • Vesicles formed during, can be extremely small
      • Exocytosis
        • Passage of material out of the cell
          • Useful secretion, as in the case of digestive enzymes, hormones or mucus

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