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  • Microscopy
    • Light
      • Specimens are illuminated with light, which is focused using glass lenses and viewed using the eyepiece. They use several lenses to obtain high magnification. Specimens need to be stained with colour stain.
        • Advantages: Specimens can be living or dead, can be cheaper than electron.
        • Disadvantages: Resolution is not very good, cells and tissues are visible but organelles cannot be seen in detail, specimen must be thin to allow light through.
    • Electron
      • SEM- Scans a fine beam of electron onto a thicker specimen. Excellent 3D images. Lower resolution than TEM.
      • TEM- transmits beams of electrons through a thin specimen. Highest resolution.


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