Pratical Skills
- Created by: 13ccooke
- Created on: 10-10-18 11:43
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- Microscopes
- Staining specimen
- Methylene
- Blue stain that can be used to stain any specimen
- Some stains bind to specific cell structures
- Acetic orecin
- Stain DNA dark red
- Gentian Violet
- Stains bacterial cell walls
- Easin
- Stains cytoplasm
- Iodine
- Stains cellulose
- Acetic orecin
- Methylene
- Artefacts
- These are substances that can be seen under a microscope but are not part of the specimen
- Cell Fractionation
- 1.keep specimen cold
- 2. Isotonic- prevent damage by osomosis
- 3.Ultracentrifugation- separting organellles
- 1. sepsarte according to size
- 2. Remove supernatant and repeat
- magnification
- How much bigger an image is compared to the oprginal specimen
- Microscopes produce linear magnification
- Resolution
- the ability of an optical intrustment to see or produce an image that shows fine detail clearly
- Staining specimen
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