Microscopy
Nearly everything needed to know on microscopy (may add more)
- Created by: desiree98
- Created on: 18-09-14 18:54
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- Microscope
- Light microscope
- Uses glass lenses to refract light rays to produce an image (magnified image)
- Must be thin (colourless), transparent and kept flat
- Magnification = 1,000s
- Stains
- Chemicals which bind to chemicals on specimen
- Methyl Blue and Iodine
- Acetic orcein - stains DNA dark red
- Gentian Violet - stains bacterial walls
- Electron
- Beams of electrons
- Transmission Electron Microscope (TEM)
- Shape + detail of smaller organelles
- Transmission Electron Microscope (TEM)
- No colour, unless added later
- Magnification = 100,000s
- Has to be dead + dried
- Has a vacuum so air particles don't interfere with electrons
- Beams of electrons
- Untitled
- Magnification
- 1mm - (x1000) = 1000 micrometer - (x1000) = 1,000,000 nanometer
- Image size (in micrometer) = magnification x actual size (in micrometer)
- I
- M
- A
- Think of the name "Mia"
- I
- Resolution
- A measure of the ability to distinguish between two different points
- Depends on the wavelength of the form of radiation used
- Light microscope
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