Methods of immobilising enzymes

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  • Immobilising enzymes
    • Absorption
      • Enzymes mixed with porous substance
      • They will bind through ionic or hydrophobic interactions
    • Covalent bonding
      • Enzymes are bound to a porous substance
      • Covalent bonds hold enzymes in place
    • Entrapment
      • Matrix of fibres (cellulose) hold enzymes in place
      • Reaction can be slow because substrate needs to get through fibres
    • Membrane separations
      • Membrane is used to separate enzyme and substrate
      • Substrate will pass by enzyme membrane and bind


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