Magnification

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  • magnification
    • lenghts
      • millimeter = 1000
      • micrometer = 1 million
      • nanometer = 1000 million
      • mm x 1000 = um
      • um x 1000 = nm
      • nm / 1000 = um
      • um / 1000 = mm
    • calculation
      • magnification = image / actual
    • microscopes
      • light microscopes
        • 0.2 um
      • electron microscopes
        • 0.1 nm
        • SEM
          • features
            • scans top of sample
            • beam of high energy electrons
            • build up 3D image
            • lower resolution power
            • slow
              • limitations
                • lower resolution power
          • limitations
          • TEM
            • features
              • sample must be thin
              • complex staining
              • 2D image
            • limitations
              • 2D image
              • complex staining
              • sample must be thin

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